Perbandingan Tingkat Kontaminasi Kultur Mycobacterium tuberculosis  pada Medium Lowenstein-Jensen dan BACTEC MGIT 960

Athira Presialia; Ariyani Kiranasari

doi:10.36452/jkdoktmeditek.v26i2.1829

Authors

Athira Presialia Fakultas Kedokteran, Universitas Indonesia, Jakarta, Indonesia
Ariyani Kiranasari Departemen Mikrobiologi Klinik, Fakultas Kedokteran Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v26i2.1829

Keywords:

BACTEC MGIT 960, Lowenstein-Jensen, Mycobacterium tuberculosis, tingkat kontaminasi

Abstract

Sampai saat ini, kultur masih merupakan standar pemeriksaan diagnosis tuberkulosis. Metode yang sering dilakukan adalah menggunakan Medium Lowenstein-Jensen dan BACTEC MGIT 960, namun kontaminasi oleh bakteri lain maupun jamur dapat terjadi dan dapat mengganggu hasil interpretasi. Penelitian ini bertujuan untuk mengetahui perbandingan tingkat kontaminasi kultur Mycobacterium tuberculosis pada Medium Lowenstein-Jensen dan BACTEC MGIT 960. Penelitian ini menggunakan desain studi potong lintang dengan 204 sampel dari Laboratorium Mikrobiologi Klinik FKUI-RSCM yang memenuhi kriteria penelitian. Data dianalisis menggunakan uji McNemar untuk data komparatif kategorik berpasangan. Dari 204 subjek yang memberikan bahan kultur, mayoritas berada pada rentang usia 26-35 tahun (34,3%) dan merupakan laki-laki (62,8%). Dari hasil uji McNemar, didapatkan tidak adanya perbedaan yang signifikan antara tingkat kontaminasi pada medium LJ dan BACTEC MGIT 960 (p=1,000). Proporsi hasil kultur positif pada medium LJ dan BACTEC MGIT 960 juga tidak menunjukkan adanya perbedaan yang signifikan (p=1,000). Tidak ada perbedaan proporsi tingkat kontaminasi pada medium LJ dan BACTEC MGIT 960.

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