Comparison of the Contamination Rate between Lowenstein-Jensen Media and BACTEC MGIT 960 as a Culture Method for Mycobacterium tuberculosis

  • Athira Presialia Faculty of Medicine, University of Indonesia
  • Ariyani Kiranasari Department of Clinical Microbiology, Faculty of Medicine, University of Indonesia
Keywords: BACTEC MGIT 960, contamination rate, Lowenstein-Jensen, Mycobacterium tuberculosis

Abstract

Culture is remaining a gold standard for tuberculosis diagnostic test. Lowenstein-Jensen and BACTEC MGIT 960 are two mediums that are recently used, however, contamination by other bacteria or fungal may interfere with the result’s interpretation. This study aims to find out the contamination rate comparison between Lowenstein-Jensen media and BACTEC MGIT 960. This is a cross-sectional study with 204 samples from the Clinical Microbiology Laboratory of FKUI-RSCM which meets the research criteria. Data were analyzed using McNemar test for paired proportion data. Out of the 204 subjects, the majority are male (62,8%) between the age group of 26-35 years (34,3%). Based on McNemar test, there is no significant difference of contamination rate between LJ media and BACTEC MGIT 960 (p=1.000). A significant difference in the proportion of positive culture result between samples cultivated with LJ media and BACTEC MGIT 960 was not shown either (p=1.000). In conclusion, there is no significant difference in contamination rate between LJ media and BACTEC MGIT 960.

References

1. World Health Organization. Global tuberculosis report 2015. Geneva: World Health Organization; 2015.
2. Mertaniasih NM, Alimsardjono L, Palilingan JF, Soedarsono. Rapid culture method using biphasic media on bacteriologic diagnosis to detect mycobacterium tuberculosis for determining pulmonary tuberculosis. Folia Medica Indonesiana. 2013;49(2):91-6.
3. Sjahrurachman A, Rintiswati N, Gartinah T, Solihin I, et al. Petunjuk teknis pemeriksaan biakan, identifikasi, dan uji kepekaan Mycobacterium tuberculosis pada media padat. Jakarta: Kementrian Kesehatan RI; 2012.
4. Kassaza K, Orikiriza P, Llosa A, Bazira J, Nyehangane D, Page AL, et al. Lowenstein-jensen selective medium for reducing contamination in Mycobacterium tuberculosis culture. J Clin Microbiol. 2014;30;52(7):2671-3.
5. Chihota VN, Grant AD, Fielding K, Ndibongo B, Zyl AV, Muirhead D, et al. Liquid vs solid culture for tuberculosis: performance and cost in a resource-constrained setting. Int J Tuberc Lung Dis. 2010;14(8):1024-31.
6. Stinson KW, Eisenach K, Kayes S, Matsumoto M, Siddiqi S, Nashima S, et al. Mycobacteriology laboratory manual. 1st ed. Geneva: WHO/GLI; 2014.
7. Somoskovi A, Magyar P. Comparison of the mycobacteria growth indicator tube with MB redox, lowenstein-jensen, and middlebrook 7H11 media for recovery of mycobacteria in clinical specimens. J Clin Microbiol. 1999;37(5):1366-9.
8. Srisuwanvilai L, Monkongdee P, Podewils LJ, Ngamlert K, Pobkeeree V, Puripokai P, et al. Performance of the BACTEC MGIT 960 compared with solid media for detection of mycobacterium in Bangkok, Thailand. Diagnostic microbiology and infectious disease. 2008;61;402-7.
9. Chien HP, Yu MC, Wu MH, Lin TP, Luh KT. Comparison of the BACTEC MGIT 960 with Lowenstein-Jensen medium for recovery of mycobacteria from clinical specimens. Int J Tuberc Lung Dis. 2000;4:866-70.
10. Siddqi SH, Rusch-Gerdes S. MGIT procedure manual for BACTEC MGIT 960 TB system. Place Unknown: Foundation for Innovative New Diagnostics; 2006.
11. Sharma M, Misra RN, Gandham NR, Jadhav SV, Angadi K, Wilson V. Comparison of modified petroff's and N-Acetyl-L-cysteine-sodium¬ hydroxide methods for sputum decontamination in tertiary care hospital in India. Med J. Dr. D.Y. Patil University. 2012;5(2):97-100.
12. Kent PT, Kubica GP. Public health mycobacteriology. A guide for the level III laboratory. Atlanta: Centers for Disease Control and Prevention; 1985.
13. Diriba G, Kebede A, Yaregal Z, Getahun M, Tadesse M, Meaza A, et al. Performance of mycobacterium growth indicator tube BACTEC 960 with lowenstein-jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian national tuberculosis reference laboratory, Addis Ababa, Ethiopia. BMC Res Notes. 2017;10(181):1-6.
14. Tripathi K, Tripathi PC, Nema S, Shrivastava AK, et al. Modified Petroff's method: an excellent simplified decontamination technique in comparison with petroff's method. Int J Recent Trends in Science and Technology. 2014;10(3):461-4.
15. Khan MS, Dar O, Tahseen S, Godfrey-Faussett P. Judging respiratory specimen acceptability for AFB microscopy: visual vs. microscopic screening. Trop Med Int Health. 2009;14:571–5. 10.1111/j.1365-3156.2009.02260.x
16. Lee J, Sou J, Lin CB, Wang JD, Lin TY, Tsai YC, et al. Comparative evaluation of the BACTEC MGIT 960 system with solid medium for identification of Mycobacterium. Int J Tuberc Lung Dis. 2003;7(6):569-74.
17. Ma Y, Fan J, Li S, Dong L, Li Y, Wang F, et al. Comparison of Lowenstein-Jensen medium and MGIT culture system for recovery of mycobacterium tuberculosis from abscess samples. Diagnostic Microbio and Infectious Disease. 2020;96(4). 10.1016/j.diagmicrobio.2019.114969.
18. Lawson L, Emenyou N, Abdurrahman ST, Lawson JO, Uzoewulu GN, Sogaolu OM, et al. Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria. BMC Res Notes. 2013;6:215.
Published
2020-06-30
How to Cite
Presialia, A., & Kiranasari, A. (2020). Comparison of the Contamination Rate between Lowenstein-Jensen Media and BACTEC MGIT 960 as a Culture Method for Mycobacterium tuberculosis. Jurnal Kedokteran Meditek, 26(2), 36-42. https://doi.org/10.36452/jkdoktmeditek.v26i2.1829
Section
Artikel Penelitian