Pemberian Hidrolisat Protein Kacang Polong Terhadap Kadar Superoksida Dismutase dan Perbaikan Kerusakan Ginjal Tikus Laboratorium

Jeanny E Ladi; David Kristiadi Herjanto; Roro Wahyudianingsih; Meilinah Hidayat

doi:10.36452/jkdoktmeditek.v28i1.2282

Authors

Jeanny E Ladi Universitas Kristen Maranatha, Bandung, Indonesia
David Kristiadi Herjanto Universitas Kristen Maranatha, Bandung, Indonesia
Roro Wahyudianingsih Universitas Kristen Maranatha, Bandung, Indonesia
Meilinah Hidayat Universitas Kristen Maranatha, Bandung , Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v28i1.2282

Keywords:

hidrolisat protein, histopatologis, kacang polong, kreatinin, SOD

Abstract

Antioksidan merupakan substansi yang dapat mencegah kerusakan sel akibat stres oksidatif. Hidrolisat protein dari kacang polong hijau (Pisum sativum) dengan bromelain (HPPHB) diharapkan meningkatkan kadar antioksidan superoxide dismutase (SOD) dan mampu mencegah kerusakan ginjal. Tujuan penelitian adalah mengetahui pengaruh HPPHB terhadap kadar SOD, kadar kreatinin plasma dan histopatologis ginjal tikus yang diinduksi Cisplatin. Penelitian dilakukan dalam dua tahap, pemberian HPPHB dosis 100, 200 dan 400 mg/kgBB/h selama 28 hari terhadap kadar SOD tikus jantan dan betina Sprague Dawley (SD) tanpa induksi dan tikus yang diinduksi Cisplatin dengan parameter kadar kreatinin plasma dan histopatologis ginjal dengan pewarnaan Hematoksilin Eosin (HE). Hasil menunjukkan kadar SOD kelompok tikus kontrol berbeda bermakna dan sangat bermakna dengan kelompok perlakuan. Hasil pemeriksaan kreatinin plasma menunjukkan terdapat perbedaan yang sangat bermakna (p<0,01) antara kontrol positip dengan kelompok dosis 400 mg/kgBB. Hasil analisis mikroskopis histopatologis parameter nekrosis: kelompok perlakuan dosis 200 menunjukkan paling sedikit nekrosis di antara ke tiga dosis, berbeda signifikan dengan kontrol positip, maupun kontrol negatip (p<0,05). Parameter densitas sel mesangium, semua kelompok dosis perlakuan tidak berbeda signifikan dengan kontrol negatip maupun kontrol positip. Simpulan, HPPHB meningkatkan kadar SOD dan menunjukkan efek perbaikan kadar kreatinin plasma dan nekrosis sel tubulus ginjal tikus SD yang diinduksi Cisplatin.

References

Levey AS, Coresh J.Chronic kidney disease. The Lancet. 2012;379(9811): 165-80.

Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. Hasil utama riset kesehatan dasar (RISKESDAS). Jakarta: Balitbangkes; 2018. p. 74–6.

Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: Harms and benefits for human health. Hindawi J. 2017;2017:1–13.

Réus GZ, Carlessi AS, Silva RH, Ceretta LB, Quevedo J. Relationship of oxidative stress as a link between diabetes mellitus and major depressive disorder. Hindawi J. 2019;2019(Mdd):1–6.

Dahl WJ, Foster LM, Tyler RT. Review of the health benefits of peas (Pisum sativum L.). Br J Nutr. 2012;108(SUPPL. 1):3–10.

Roy F, Boye JI, Simpson BK. Bioactive proteins and peptides in pulse crops: Pea, chickpea and lentil. Food Res Int. 2010;43(2):432–42.

Stanisavljević NS, Vukotić GN, Pastor FT, Sužnjević D, Jovanović ŽS, Strahinić ID, et al. Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. Arch Biol Sci. 2015;67(3):1033–42.

Hidayat M. Hidrolisat protein dari kacang polong hijau (Pisum sativum, L) untuk penyakit ginjal kronis. Bandung: Penerbit Alfabeta; 2018.p 1–262.

Pownall TL, Udenigwe CC, Aluko RE. Amino acid composition and antioxidant properties of pea seed (Pisum sativum L.) enzymatic protein hydrolysate fractions. J Agric Food Chem. 2010;58(8):4712–8.

Udenigwe CC, Aluko RE. Food protein-derived bioactive peptides: Production, processing, and potential health benefits. J Food Sci. 2012;77(1):11–24.

Phan TT, Lim I, Chan S, Tan EK, Lee ST, Longaker M. Suppression of transforming growth factor beta/smad signaling in keloid-derived fibroblasts by quercetin: Implications for the treatment of excessive scars. The Journal of Trauma. 2004;57(5):1032-7.

Avila-Carrasco L, Majano P, Sánchez-Toméro JP,Selgas R, López-Cabrera M, Aguilera A, et al.. Natural plants compounds as modulators of epithelial-to-mesenchymal transition. Front Pharmacol. 2019;10(175).

Hidayat M, Prahastuti S, Wargasetia T, Nugraha K, Soemardji A, Rahmawati S, et al. Green peas protein hydrolyzed by bromelain in simple procedure to improve kidney function in cisplatin-induced rats. J Reports Pharm Sci. 2019;8(1):68–77.

Hidayat M, Prahastuti S, Wahyudianingsih R, Wargasetia TL, Ferdinand V, Soemardji AA, et al. Role of pea protein hydrolysates as anti-nephrotoxicity. J Pharm Res Sci. 2019; 8:55-60.

Abcam. Superoxide dismutase activity assay kit (colorimetric). 2019. Available from: https://www.abcam.com/superoxide-dismutase-activity-assay-kit-colorimetric-ab65354.html

Ajibola CF, Fashakin JB, Fagbemi TN, Aluko RE. Effect of peptide size on antioxidant properties of African yam bean seed (Sphenostylis stenocarpa) protein hydrolysate fractions. International Journal of Molecular Sciences. 2011;12(10):6685-702.

Pergola PE, Raskin P, Toto RD, Meyer CJ, Huff JW, Grossman EB, et al. BEAM study investigators. Bardoxolone methyl and kidney function in CKD with type 2 diabetes. N Engl J Med. 2011;365(4):327-36.

Gewin LS. Renal fibrosis: Primacy of the proximal tubule. Matrix Biol. 2018;68-69:248-62.

Berchtod L, Friedli I, Vallée JP, Moll S, Martin PY, de Seigneux S. Diagnosis and assessment of renal fibrosis: the state of the art. Swiss Med Wkly. 2017;147: w14442.

Hidayat M, Prahastuti S, Riyani DU, Soemardji A, Hasan K. Kidney therapeutic potential of peptides derived from the bromelain hydrolysis of green peas protein, Iranian Journal of Basic Medical Sciences, 2019;22: 1016-25.

Zheng XN, Wang XW, Li LY, Xu ZW, Huang HY, Zhao JS, et al. Puerh tea powder preventive effects on cisplatin-induced liver oxidative damage in Wistar rats. Asian Pac J Cancer Prev. 2014;15(17):7389-94.

Tatli SP, Seven I, Karakus S, Iflazoglu MS, Arkali G, Muge Sahin Y, et al. Turkish propolis and its nano form can ameliorate the side effects of cisplatin, which is a widely used drug in the treatment of cancer. Plants (Basel). 2020;9(9):1075.