Expression of Nuclear Factor – kappa B (NF-kB) in Human Breast Cancer Stem Cells (CD 24-/CD 44+) Treated with H2O2 and Its Relationship  with Cell Viability

Hendrik Kurniawan; Septelia Inawati  Wanandi; Sri Widia A  Jusman

doi:10.36452/jkdoktmeditek.v29i2.2668

Authors

Hendrik Kurniawan Biochemistry and Biomolecular Department, Faculty Medicine and Health Sciences, Krida Wacana Christian University, Jakarta, Indonesia
Septelia Inawati Wanandi Biochemistry and Biomolecular Department, Faculty Medicine, Indonesia University, Jakarta, Indonesia
Sri Widia A Jusman Biochemistry and Biomolecular Department, Faculty Medicine, Indonesia University, Jakarta, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v29i2.2668

Keywords:

antioxidant, cancer stem cells, H2O2, NF-kB, oxidative stress

Abstract

Introduction: Breast cancer is one of the highest causes of death from cancer in women in Indonesia. This is partly due to the resistance of ROS-based therapies such as radiotherapy and chemotherapy. Breast cancer stem cells (cancer stem cells, CSCs) have a role in this resistance mechanism. Previous studies demonstrated the ability of CSC to survive oxidative stress conditions due to rotenone administration. Therefore, in this study an analysis was carried out on the transcription factor NF-kB in breast cancer cells, both CSCs and Non CSCs, related to the role of NF-kB in maintaining the survival of cancer cells under conditions of oxidative stress. Methods: The study was conducted on human breast cancer stem cells (CD24-/CD44+) and non stem cells (CD24-/CD44-) which were given H₂O₂ at concentrations of 1.1µM, 11µM, and 110µM with control cells not given H₂O₂. Assessment was carried out on the parameters of NF-kB mRNA expression, and cell viability. Results: Administration of H₂O₂ at a concentration of 11µM showed a significant increase in the expression of NFk-B CSCs mRNA compared to non CSCs (p<0.05). As for the viability test results, at all concentrations of H₂O₂ it appears that CSCs was able to maintain its viability compared to non CSCs which experienced a decrease in viability (p<0.05). Conclusion: In this study, conditions of oxidative stress due to the administration of H₂O₂ led to an increase in the expression of NF-kB mRNA in CSCs so that cell viability could be maintained.

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