Function of Moringa oleifera Lamk Leaf Extract as an Antiseptic for  Pseudomonas aeruginosa and Staphylococcus aureus using Percentage Kill Method

Ariyani Kiranasari; Conny Riana  Tjampakasari; Mariza Nabila Putri; Vanessa Maritza  Pratama

doi:10.36452/jkdoktmeditek.v30i3.2864

Authors

Ariyani Kiranasari Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia, Jakarta, Indonesia
Conny Riana Tjampakasari Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia, Jakarta, Indonesia
Mariza Nabila Putri Fakultas Kedokteran, Universitas Indonesia, Jakarta, Indonesia
Vanessa Maritza Pratama Fakultas Kedokteran, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v30i3.2864

Keywords:

moringa oleifera leaf , pseudomonas aeruginosa, staphylococcus aureus, percentage kill

Abstract

Moringa oleifera Lamk leaf extract has an antimicrobial properties especially against fungal, parasite, Gram positive and negative bacteria by inhibiting DNA synthesis and metabolism an damaging cell walls. The aim of this study was to determine whether M. oleifera Lamk leaf extract is effective as an antiseptic against P. aeruginosa and S. aureus bacteria. Percentage Kill was used in the study to determine the percentage of bacteria death after contact with M. oleifera Lamk leaf extract in the 1^st, 2^ndand 5^th minutes against the control and treatment simultaneously. The test is considered to meet the standard if each contact gives a result of ≥90%. For discovered percentage kill to P. aeruginosa bacteria in the 1^st, 2^ndand 5^th minutes each test showed a yield of 27.12%. , 47.01%, 57.7%. In the third time of P. aeruginosa did not reach the standard. Whereas in S. aureus bacteria, each test showed a yield of 92.36%, 95.58% and 96.45%, where it was seen that all results reach the standards criteria of ≥90%. M. oleifera Lamk leaf extract was not effective in eliminating P. aeruginosa for all contacts because the standard value was below 90%. Whereas for S. aureus bacteria, M. oleifera Lamk leaf extract was very effective in eliminating bacteria for all contact times ≥90% with the highest value at the 5^th minutes (96.45%).

References

Vergara-Jimenez M, Almatrafi MM, Fernandez ML. Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants. 2017;6(4):91.

World Health Organization. Disinfectants and antiseptics. Geneva: World Health Organization; 2004.

Wu W, Jin Y, Bai F, Jin S. Pseudomonas aeruginosa. Molecular medical microbiology. Academic Press; 2015. pp. 753-767).

Spagnolo AM, Sartini M, Christina ML. Pseudomonas aeruginosa in the healthcare facility setting. Reviews in Medical Microbiology. 2021;32:169–175.

Taylor TA. Staphylococcus Aureus. US National Library of Medicine. 2020. Cited 2021 Feb 12. Available from: https://www.ncbi.nlm.nih.gov/books/NBK441868/

Brugger SD, Baumberger C, Jost M, Jenni W, Brugger U, Mühlemann K. Automated counting of bacterial colony forming units on agar plates. PLoS One. 2012;7(3):e33695.

Mahon CR, Lehman DC, Manusellis G. Textbook of diagnostic microbiology. 6th ed. USA: WB. Saunders; 2019.

Capuccino JG, Sherman N. Microbiology a laboratory manual. 12ed. State University of New York: Pearson Benjamin Cummings; 2020. p. 93 – 135.

Conny RT. Operational procedure : Percentage kill. Microbiology clinical laboratory. Jakarta: Universitas Indonesia; 2022.

Jan BS. Standard guide for assessment of microbial activity using a time-kill procedure. Texas: Microchem Laboratory; 2019.

Farmakope Indonesia. 6th edition. Jakarta: Ministry of Health of The Republic Indonesia. Jakarta; 2020.

Rachmawati SR. Characterization of Moringa (Moringa Oleifera Lam.) leaf water extracts by chemical and microbiology. SANITAS J Teknol Dan Seni Kesehat. 2019;10(2):102–16

Widowati I, Efiyati S, Wahyuningtyas S. Uji aktivitas antibakteri ekstrak daun kelor (Moringa oleifera) terhadap bakteri pembusuk ikan segar (Pseudomonas aeruginosa). Pelita-Jurnal Penelitian Mahasiswa UNY. 2014;9(02).

Raubilu IA, Isah U, Ahmad MA. Antimicrobial activity of Moringa oleifera: A short review. Bayero Journal of Pure and Applied Sciences. 2019;12(1):128-32.

Dewangan G, Koley KM, Vadlamudi VP, Mishra A, Poddar A, Hirpurkar SD. Antibacterial activity of Moringa oleifera (drumstick) root bark. Journal of Chemical and Pharmaceutical Research. 2010;2(6):424-8.

Abdalla M, Hastings A, Truu J, Espenberg M, Mander U, Smith P . Emissions of methane from northern peatlands: a review of management impacts and implications for future management options. Ecol Evol. 2016;6(19):7080-7102.

Wajdi SA, Kasmiyati S, Hastuti SP. Uji aktivitas antibakteri campuran ekstrak biji kelor (Moringa oleifera) dan daun kersen (Muntingia calabura) terhadap Pseudomonas aeruginosa dan Bacillus subtilis. Journal of Tropical Biodiversity and Biotechnology. 2017;2(1):10-5.

Isenberg HD, Garcia LS. Clinical microbiology procedures handbook. 3rd edition. Washington DC; 2015(1).

Subhan A, Manalu W, Rahminiwati M. Inovasi formula produk hand rub berbasis alkohol sebagai upaya efisiensi pengelolaan sediaan farmasi di Rumah Sakit. Majalah Farmasetika. 2019;4 (Suppl 1):256 – 262.

Yagnik D, Ward M, Shah AJ. Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli. Scientific Reports. 2021;11(1):1-7.

Buldain D, Castillo LG, Marchetti ML, Lozano KJ, Bandoni A, Mestorino. N. Modeling the Growth and Death of Staphylococcus aureus against Melaleuca armillaris essential oil at different pH Conditions. Antibiotics. 2021;10(222).

Kou X, Li B, Olayanju JB, Drake JM, Chen N. Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients. 2018;10(3):343.

Ginarana A, Warganegara E, Oktafany O. Uji aktivitas antibakteri formulasi gel ekstrak daun kelor (Moringa oleifera) terhadap Staphylococcus aureus. Jurnal Majority. 2020;9(2).

Wigunarti AH, Pujiyanto S, Suprihadi A. Uji aktivitas antibakteri ekstrak biji kelor (Moringa oleifera L.) terhadap pertumbuhan bakteri Staphylococcus aureus dan bakteri Escherichia coli. Berkala Bioteknologi. 2019;2(2).