Uji Toksisitas Obat dengan Larva Ikan Zebra

  • Susana Elya Sudradjat Department of Pharmacyst, Faculty of Medicine, Krida Wacana Christian University
Keywords: kardiotoksisitas, hepatoktoksisitas, neurotoksisitas, toksisitas, larva ikan zebra

Abstract

Toksisitas adalah salah satu masalah utama dalam proses pengembangan obat. Kardiotoksisitas, neurotoksisitas dan hepamerupakan alasan utama obat tidak lolos dalam uji klinik atau ditarik dari pasaran. Larva ikan zebra dapat menjembatani antara uji in vitro dan model hewan mamalia mengerat dengan lebih cepat dan ekonomis. Toksisitas organ dapat dideteksi mulai pada tahap larva sehingga dapat diprediksi dengan tepat pengaruh obat tersebut pada manusia. Uji kardiotoksik, neurotoksik dan hepatotoksik dapat dilakukan pada hewan yang sama dengan ketepatan dan ketelitian yang tinggi.

 

 

References

1. Legradi J, El Abdellaoui N, Van Pomeren M, Legler J. Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity. Environ. Sci. Pollut. Res. 2014;22, 16277–89.
2. European Union. European Union Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. (2010). Off. J. Eur. Union. 2010;33–79.
3. Gaikwad S, et al. Constructing the habituome for phenotype-driven zebrafish research. Behav Brain Res. 2012;236:110–7.
4. Calum AMR, Randal TP. Zebrafish as tools for drug discovery. Nature Reviews Drug Discovery. 2015.14:721–31.
5. Santoriello C, Zon LI, Santoriello C, Zon LI. Hooked ! Modeling human disease in zebrafish Find the latest version : Science in medicine Hooked ! Modeling human disease in zebrafish. 2012;122(7):2337–43.
6. Hermsen SAB, van den Brandhof EJ, van der Ven LTM, Piersma AH. Relative embryotoxicity of two classes of chemicals in a modified zebrafish embryotoxicity test and comparison with their in vivo potencies. Toxicol Vitr. 2011;25(3):745–53.
7. JörgensK, Hillebrands JL, Hammes HP, KrollJ. Zebrafish: A model for understanding diabetic complications. Experimental and Clinical Endocrinology and Diabetes. 2012;120(4):186–7.
8. Chongjun Z, et al. Zebrafish model for assessing induced organ toxicity by Strychnos nux-vomica. J Tradit Chinese Med. 2016;36(4):522–9.
9. Kanungo J, Cuevas E, Ali SF, Paule MG. Zebrafish Model in Drug Safety Assessment. 2014;5416–29.
10. Hartmann S, et al. Zebrafish larvae show negative phototaxis to near-infrared light. PLoS One. 2018;13(11):1–16.
11. Cornet C, et al. ZeGlobalTox: An innovative approach to address organ drug toxicity using zebrafish. Int. J. Mol. Sci. 2017;18:1-19.
12. Ota S, Kawahara A. Zebrafish: A model vertebrate suitable for the analysis of human genetic disorders. Congenit Anom (Kyoto). 2014;54(1):8–11.
13. Asnani A, Peterson RT. The zebrafish as a tool to identify novel therapies for human cardiovascular disease. Dis Model Mech. 2014;7(7):763–7.
14. Dhillon SS, et al. Optimisation of Embryonic and Larval ECG Measurement in Zebrafish for Quantifying the Effect of QT Prolonging Drugs. PLoS ONE. 2013 8: e60552.
15. Vliegenthart ADB, Tucker CS, Del Pozo J, Dear JW. Zebrafish as model organisms for studying drug-induced liver injury. Br J Clin Pharmacol. 2014;78(6):1217–27.
16. Sarras Jr MP. Genetic and chemically-induced Zebrafish models for the study of diabetes mellitus. MOJ Anat Physiol. 2018;5(5):319–21.
17. Goessling W, Sadler KC. Zebrafish: An Important Tool for Liver Disease Research. Gastroenterology. 2015;149(6):1361–77.
18. Howarth DL, Yin C, Yeh K, Sadler KC. Defining hepatic dysfunction parameters in two models of fatty liver disease in zebrafish larvae. Zebrafish.2013.10:199–210.
19. Tavares B, Santos Lopes S. The importance of Zebrafish in biomedical research .Acta Med Port. 2013;26(5):583–92.
20. Quinlivan VH, Farber SA, Michel M, Farber SA. Lipid Uptake , Metabolism , and Transport in the Larval Zebrafish. 2017;8(November):1–11.
21. Passeri MJ. Hepatic Steatosis in Response to Acute Alcohol Exposure in Zebrafish requires Srebp Activation. 2010;49(2):443–52.
22. Ahmad F, Noldus LPJJ, Tegelenbosch RAJ, Richardson MK. Zebrafish embryos and larvae in behavioural assays. 2012;149:1241–81.
23. Outeiro TF. Green fluorescent protein labeling of dopaminergic neurons in zebrafish for the study of parkinson’s diseases. J Microbiol Exp. 2017;4(1)
24. Blazina AR, Vianna MR, Lara DR. The Spinning Task: A new protocol to easily assess motor coordination and resistance in zebrafish. Zebrafish. 2013;10(4):480–5.
25. Afrikanova T, et al. Validation of the zebrafish pentylenetetrazol seizure model: Locomotor versus electrographic responses to antiepileptic drugs. PLoS One. 2013;8(1):1–9.
26. Maximino C, et al. Measuring anxiety in zebrafish: A critical review. Behav Brain Res. 2010;214(2):157–71.
27. Liu C, Zhou J. Validation of the zebrafish pentylene tetrazol seizure model: Behaviour assay for assessing anti-epileptic drug efficacy. Biochem Anal Biochem. 2016;5(2).
Published
2019-09-06
How to Cite
Sudradjat, S. (2019). Uji Toksisitas Obat dengan Larva Ikan Zebra. Jurnal Kedokteran Meditek, 25(2), 88-93. https://doi.org/10.36452/jkdoktmeditek.v25i2.1749
Section
Tinjauan Pustaka