Uji Fenotipik Deteksi Enzim ESBL, AmpC dan Karbapenemase pada Bakteri Enterobacteriaceae
DOI:
https://doi.org/10.36452/jkdoktmeditek.v25i3.1754Keywords:
Enterobacteriaceae, ESBL, AmpC, CarbapenemaseAbstract
Organisms that express extended-spectrum β-lactamase (ESBLs) such as Enterobacteriaceae have been increasing for the past forty years. Organisms that express this enzyme can hydrolyze oxyminocephalosporins and monobactam,but can be inhibited using β-lactam inhibitors. There are various types of enzymes that contribute to cephalosporin and monobactam resistances, such as AmpC β-lactamase, and carbapenemase enzymes. Polymerase Chain Reaction (PCR) is an accurate method for detecting ESBL, but this method is a genotypic method that requires a specialized instrument. Therefore there is a need for a phenotypic method to detect ESBL, AmpC and Carbapenemase that can be conducted in various laboratories.
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3. Naelasari DN, Koendhori EB, Dewanti L, Sarassari R, Kuntaman K, Airlangga U. The prevalence of extended spectrum beta-lactamase (ESBL) producing gut bacterial flora among patients in Dr . Soetomo Hospital and primary health centers in Surabaya. Folia Medica Indones. 2018;54(4):256-62.
4. Tille PM. Bailey & Scott’s Diagnostic Microbiology. 13th ed. Elsevier; 2014.
5. Ghafourian S, Sadeghifard N, Soheili S, Sekawi Z. Extended spectrum beta-lactamases: definition, classification and epidemiology. Curr Issues Mol Biol. 2015:11-22.
6. Murray PR, Rosenthal KS, Pfaller MA. Medical microbiology. 8th ed. Elsevier Inc.; 2016.
7. Clinical and Laboratory Standard Institute. M100 Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standard Institute; 2018.
8. Noël A, Huang T, Berhin C, et al. Comparative evaluation of four phenotypic tests for detection of carbapenemase-producing gram-negative bacteria. J Clin Microbiol. 2017;55(2):510-58.
9. Ruppelt A, Makarewicz O, Braun SD, et al. Rapid identification of carbapenemase genes in gram-negative bacteria with an oligonucleotide microarray-based assay. PLoS One. 2014;9(7). doi:10.1371/journal.pone.0102232
10. Tsakris A, Poulou A, Bogaerts P, Dimitroulia E, Pournaras S, Glupczynski Y. Evaluation of a new phenotypic OXA-48 disk test for differentiation of OXA-48 carbapenemase-producing enterobacteriaceae. J Clin Microbiol. 2015;53(4):1245-51. doi:10.1128/JCM.03318-14
11. Bennet JO, Dollin R, Blaser MJ. Infectious Diseases. 8th ed. Elsevier Inc. 2015(1).
12. Jacoby GA. AmpC beta-lactamases. Clin Microbiol Rev. 2009;22(1):161-82. doi:10.1128/CMR.00036-08
13. Drieux-rouzet L, Golmard J, Jarlier V. Comparison of nine phenotypic methods for detection of extended-spectrum beta-lactamase production by enterobacteriaceae. J Clin Microbiol. 2011;49(3):1048-57. doi:10.1128/JCM.02130-10
14. Khari M, Izzati F, Karunakaran R; Rosli R; Tee Tay S. Genotypic and phenotypic detection of AmpC β-lactamases in Enterobacter spp. isolated from a teaching hospital in Malaysia. PLoS One. 2016:1-12.
15. Rand KH, Turner B, Seifert H, Hansen C, Johnson JA, Zimmer A. Clinical laboratory detection of AmpC beta-lactamase: does it affect patient outcome? Am J Clin Pathol. 2011;(Jan):572-6. doi:10.1309/AJCP7VD0NMAMQCWA
16. Birgy A, Bidet P, Genel N, Doit C, Decre D, Arlet G, Bingen E. Phenotypic screening of carbapenemases and associated β-lactamases in carbapenem-resistant Enterobacteriaceae.pdf. J Clin Microbiol. 2012:1295-1302.
17. Helmy MM, Wasfi R. Phenotypic and molecular characterization of plasmid mediated AmpC β-lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis isolated from urinary tract infections in Egyptian hospitals. Biomed Res Int. 2014:1-6. doi:http://dx.doi.org/10.1155/2014/171548
18. Lutgring JD, Limbago BM. The problem of carbapenemase-producing-carbapenem-resistant- enterobacteriaceae detection. 2016;54(3):529-534. doi:10.1128/JCM.02771-15.Editor
19. Tan TY, Siew L, Ng Y, He J, Koh TH, Hsu LY. Evaluation of screening methods to detect plasmid-mediated AmpC in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Antimicrob Agents Chemother. 2009;53(1):146-9. doi:10.1128/AAC.00862-08
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Published
2019-12-23
How to Cite
Dharmawan, A., & Layanto, N. (2019). Uji Fenotipik Deteksi Enzim ESBL, AmpC dan Karbapenemase pada Bakteri Enterobacteriaceae. Jurnal Kedokteran Meditek, 25(3), 100–106. https://doi.org/10.36452/jkdoktmeditek.v25i3.1754
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