Isolasi Methicillin Resistant Staphylococcus aureus pada Mikrofon yang Digunakan saat Aktivitas Perkuliahan di Fakultas Kedokteran dan Ilmu Kesehatan Ukrida

Donna Mesina Pasaribu; Amelia Graciella Tjiptabudy; Kris Herawan Timotius

doi:10.36452/jkdoktmeditek.v26i3.1892

Authors

Donna Mesina Pasaribu Departmen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Kristen Krida Wacana, Jakarta, Indonesia
Amelia Graciella Tjiptabudy Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia
Kris Herawan Timotius Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v26i3.1892

Keywords:

microphone, Methicillin Resistant Staphylococcus aureus, UkridaFaculty of medicine and Health Science, PBP2a, mecA

Abstract

Microphone is an audio tool that is generally used during lecture. Microphone is not only used by one individual, but also by many people, a potential source of bacterial spread. The purpose of this study was to determine the presence or absence of Methicillin Resistant Staphylococcus aureus in the microphones used at the Faculty of Medicine and Health Sciences, Krida Wacana Christian University (FKIK UKRIDA). Research was conducted by using a descriptive study method with a total sampling technique. Samples taken in 2019 were all microphones used in the lecture process at the FKIK UKRIDA. A total of 26 microphones were examined by means of performing a swab on each microphone and planted on mannitol salt agar media, then transferred to nutrient agar. It was then followed by a catalase testing, gram staining, mannitol sugar testing, and sensitivity test of Staphylococcus aureus bacteria against oxacillin antibiotics. Results showed that 11 microphones contained Staphylococcus aureus bacteria and, on the sensitivity test results, all bacteria grown were sensitive to oxacillin antibiotics. The study concludes that no Methicillin Resistant Staphylococcus aureus found on the microphones used during lecture activities in FKIK UKRIDA.

References

1. Mutsaqof AAN, Wiharto STM, Suryani E. Sistem pakar untuk mendiagnosis penyakit infeksi menggunakan forward chaining. Jurnal Itsmart. 2015;4(1): 43.
2. Pasaribu, D. Pencegahan infeksi nosokomial dan resistensi antibiotika. Jurnal Kedokteran Meditek, 2014; 16(42A).
3. Gaidaka CS, Pasaribu DMR. Identifikasi Staphylococcus aureus pada tombol elevator gedung baru kampus fakultas kedokteran universitas kristen krida wacana. Jurnal Kedokteran Meditek. 2017; 23(62): 21-8.
4. Putri DA, Roebiakto E, Dwiyanti RD. Staphylococcus aureus pada keyword di unit pelayanan penunjang rumah sakit. Jurnal Skala Kesehatan Politeknik Kesehatan Banjarmasin. 2018;9(1):12-15.
5. Murwani S. Dasar–dasar mikrobiologi veteriner. Malang: Universitas Brawijaya Press; 2015: h. 164- 8.
6. Simoes RR, deSousa MA, Conceicao T, Antunes F, daCosta PM, deLencastre H. High prevalance of mrsa-15 in portuguese public buses: a worrisome finding. Journals Plos Ones. 2011;6(3):1-5.
7. Conceicao T, Diamantino F, Coelho C, deLacastre H, deSousa MA. Contamination of public buses with mrsa in lisbon, portugal: a possible transmission route of major mrsa clones within the community. Journals Plot One. 2013;8(11):1-5.
8. Dellit T, Duchin J, Hofmann J, Olson EG. Interim guidelines for evaluation & management of community associated Methicillin resistant Staphylococcus aureus skin and soft tissue infection in outpatient settings. Washington: Infection Disease Society of Washington. 2007:1-14.
9. Tille PM. Bailey & Scotts’sdiagnostiv microbiology thirdteenth edition. St. Louis, Missouri: Elsevier Mosby. 2014:232-45.
10. Kemalaputri DW, Jannah SN, Budiharjo A. Deteksi MRSa (Methicillin Resistant Staphylococcus aureus) pada pasien rumah sakit dengan metode maldi-tofms dan multiplex PCR. Jurnal Biologi. 2017;6(4):51-61.
11. Pasaribu DMR. Konsentrasi hambatan minimal moxyfloxacin dan ciprofloxacin pada methicillin resisten Staphylococcus aureus dan metisilin aensitif Staphylococcus aureus. Jurnal Kedokteran Meditek. 2011;17(43):1-9
12. Dewi AK. Isolasi, identifikasi dan uji sensitivitas Staphylococcus aureus terhadap amoxicillin dari sampel susu kambing peranakan ettawa (pe) penderita mastitis di wilayah Girimulyo, Kulonprogo, Yogyakarta. Jurnal Sain Veteriner. 2013;31(2):138-150.
13. Widianingsih M, Setyorini DC. Identifikasi Staphylococcus aureus pada abonsapi di pasar pahingkota Kediri. Bioeksperimen. 2019;5(2):99-105.
14. CLSI. Breakpoints Eliminated from CLSI document M100 Since 2010. Diunduh dari https://clsi.org/media/1828/_m100_archived_drugs_table.pdf, 20 Januari 2020.
15. Rahmi Y, Darmawi, Abrar M, Jamin F, Fakhrurrazi, Fahrimal Y. Identifikasi bakteri Staphylococcus aureus pada preputium dan vagina kuda (Equus caballus). Jurnal Medika Veterinaria. 2016;9(2):154-7.
16. Harianih AM. Analisis densitas dan diversitas bakteri pada permukaan mikrofon studio music di kota Yogyakarta, Daerah Istimewa Yogyakarta. Dinduh dari http://etd.repository.ugm.ac.id/, 3 Desember 2019.
17. Giuseppe I, Sebastiano Leone, Francesco NL, Emanuele Nicastri, Richard PW. Methicillin-resistant Staphylococcus aureus: the superbug. International Journal of Infectious Diseases. 2010;14S4: S7–S11
18. Sudigdoadi S. Analisis tipe staphylococcal chromosome mec (SCCmec) isolate Methicillin Resistant Staphylococcus aureus (MRSa). MKB. 2010;42(4):149-54.
19. Centre for Disease Control and Prevention. When and how to wash your hands. Diunduh dari https://www.cdc.gov/handwashing/when-how-handwashing.html, 20 Januari 2020.
20. Susastyo JH. Perbedaan pengaruh pengolesan dan perendaman alkohol 70% terhadap penurunan angka hitung kuman pada alat kedokteran gigi. Jurnal Vokasi Kesehatan. 2016;2(2):160-4.