Potensi Hibiscus sabdariffa Linn. Memodulasi GLP-1 dan Neuropeptide Y (NPY) di Nucleus Arcuatus pada Tikus Obes

Rickie; Neng Tine  Kartinah; Minarma   Siagian; Nurhadi  Ibrahim; Suci  Anggraini

doi:10.36452/jkdoktmeditek.v28i2.2346

Authors

Rickie Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia
Neng Tine Kartinah Departemen Fisiologi Kedokteran, Fakultas Kedokteran Universitas Indonesia, Jakarta, Indonesia
Minarma Siagian Departemen Fisiologi Kedokteran, Fakultas Kedokteran Universitas Indonesia, Jakarta, Indonesia
Nurhadi Ibrahim2 Departemen Fisiologi Kedokteran, Fakultas Kedokteran Universitas Indonesia, Jakarta, Indonesia
Suci Anggraini Program Magister Ilmu Biomedis, Fakultas Kedokteran Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v28i2.2346

Keywords:

Glucose like Peptide -1 (GLP-1), Hibiscus sabdariffa Linn. (HSL), Neuropeptide Y (NPY), Nucleus Arcuatus

Abstract

Gangguan pada pusat pengaturan nafsu makan dapat menyebabkan obesitas. Glucagon like Peptide -1 (GLP-1) dan Neuropeptide Y (NPY) merupakan dua peptida penting yang terlibat dalam pengendalian nafsu makan. Beberapa penelitian menunjukkan Hibiscus sabdariffa Linn. memiliki potensi dalam pengendalian obesitas dengan memodulasi kadar GLP-1 dan NPY di perifer. Penelitian ini bertujuan untuk mengetahui bagaimana potensi Hibiscus sabdariffa Linn.(HSL) dalam memodulasi kadar GLP-1 dan NPY di sentral khususnya di Nucleus Arcuatus. Penelitian ini merupakan penelitian lanjutan yang menggunakan jaringan otak tikus tersimpan yang berasal dari 24 ekor tikus Spraque Dawley jantan umur 6 -10 minggu, berat badan antara 90 -160 gram, yang dibagi dalam 4 kelompok yaitu: Normal(C); Obes(Ob); Obes-Hib200(Ob-Hib200); Obes-Hib400(Ob-Hib400). Ekstrak HSL diberikan secara oral satu kali per hari selama 5 minggu. Sampel diperoleh dari hasil isolasi ARC, kemudian dilakukan pengukuran kadar GLP-1 dan NPY menggunakan metode ELISA. Hasil penelitian menunjukkan ekstrak HSL dosis 400mg/KgBB/hari dapat meningkatkan kadar GLP-1 paling tinggi, dibandingkan dengan kelompok tikus lainnya, sekaligus dapat menurunkan kadar NPY dibandingkan dengan kelompok tikus Obes(Ob) dan Obes-Hib(200), meskipun secara analisis tidak terdapat perbedaan bermakna. Dosis ekstrak HSL berperan penting dalam efektifitasnya memodulasi kadar GLP-1 dan NPY di ARC untuk membantu mengendalikan nafsu makan sehingga dapat mengurangi resiko terjadinya obesitas.

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