The Kemampuan Ekstrak Etanol Batang Brotowali (Tinospora crispa L.) Sebagai Antifungi Terhadap Candida tropicalis

Suryani Hutomo; Ceny Gloria  Larope; Christiane Marlene  Sooai; Maria Silvia Merry

doi:10.36452/jkdoktmeditek.v29i2.2622

Authors

Suryani Hutomo Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Kristen Duta Wacana, Yogyakarta, Indonesia
Ceny Gloria Larope Fakultas Kedokteran, Universitas Kristen Duta Wacana, Yogyakarta, Indonesia
Christiane Marlene Sooai Departemen Parasitologi, Fakultas Kedokteran, Universitas Kristen Duta Wacana, Yogyakarta, Indonesia
Maria Silvia Merry Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Kristen Duta Wacana, Yogyakarta, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v29i2.2622

Keywords:

Antifungi, brotowali, Candida tropicalis, konsentrasi hambat minimum, Tinospora crispa L

Abstract

Candida tropicalis adalah salah satu spesies non-Candida albicans yang paling umum diisolasi dari berbagai jenis kandidiasis dan infeksi nosokomial. Spesies ini adalah yang paling virulen kedua di antara spesies Candida. Meluasnya penggunaan antijamur sebagai profilaksis menjadi penyebab utama resistensi antifungi. Batang brotowali (Tinospora crispa L.) digunakan sebagai obat herbal di Asia, termasuk Indonesia. Terdapat lebih dari 65 senyawa fitokimia, dan beberapa di antaranya diidentifikasi sebagai agen antijamur potensial, seperti kelompok flavon, alkaloid, berberin, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur batang brotowali dan menentukan konsentrasi hambat minimum. Batang brotowali terstandar diolah dengan metode maserasi untuk menghasilkan ekstrak etanol. Kerentanan C. tropicalis terhadap ekstrak etanol batang brotowali diperiksa dengan uji konsentrasi hambat minimum menggunakan metode mikrodilusi dengan konsentrasi awal 10.000 µg/ml. Hal ini menunjukkan bahwa nilai KHM ekstrak etanol batang brotowali berada pada konsentrasi 5.000 µg/ml. Sebagai kesimpulan, ekstrak etanol batang brotowali memiliki aktivitas antifungi yang lemah terhadap C. tropicalis dengan konsentrasi hambat minimal 5.000 µg/ml.

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