Pengaruh Spirulina platensis terhadap Aktivitas Katalase Hepar Tikus (Rattus Norvegicus) yang Diinduksi Parasetamol Dosis Tinggi

Sindy Arum Hapsari; Sihning E. J. T.; Rizqi Rokhmadhoni Pikir; Djatiwidodo Edi Pratiknya

doi:10.36452/jkdoktmeditek.v29i3.2758

Authors

Sindy Arum Hapsari Fakultas Kedokteran, Universitas Hang Tuah, Surabaya, Indonesia
Sihning E. J. T. Departemen Histologi, Fakultas Kedokteran, Universitas Hang Tuah, Surabaya, Indonesia
Rizqi Rokhmadhoni Pikir Departemen Anak, Fakultas Kedokteran, Universitas Hang Tuah, Surabaya, Indonesia
Djatiwidodo Edi Pratiknya Departemen Kelautan, Fakultas Kedokteran, Universitas Hang Tuah, Surabaya, Indonesia

DOI:

https://doi.org/10.36452/jkdoktmeditek.v29i3.2758

Keywords:

Antioksidan, Katalase, Rattus norvegicus, Spirulina platensis

Abstract

Drug-Induced Liver Injury (DILI), seringkali disebabkan oleh akumulasi metabolit reaktif yang dihasilkan obat seperti parasetamol, N-acetyl-p-benzoqui-none imine (NAPQI) sehingga memicu terjadinya stres oskidatif, yaitu kondisi dimana perbandingan jumlah radikal bebas yang lebih tinggi daripada jumlah antioksidan. Ekstrak Spirulina platensis (S. platensis) yang kaya antioksidan berpotensi menangkal radikal bebas. Peneliti terdahulu menunjukkan S. platensis 360 mg/KgBB dapat menurunkan malondialdehida sebagai indikator kadar radikal bebas. Oleh karena itu, penelitian ini dilakukan uji aktivitas katalase untuk mengetahui peran S. platensis 360 mg/KgBB sebagai indikator kadar antioksidan pada kondisi stres oksidatif. Penelitian dilakukan dengan randomized posttest only control group design. Sampel sebanyak 30 ekor tikus Rattus norvegicus jantan, dibagi menjadi 3 kelompok: (K-) kelompok yang tidak mendapat perlakuan; (K+) kelompok yang diinduksi parasetamol; (Kp) kelompok yang diinduksi parasetamol dan diberi ekstrak S. platensis 360 mg/KgBB. Pada akhir penelitian, seluruh kelompok dilakukan perhitungan aktivitas katalase jaringan hepar. Hasil uji One Way Anova menunjukkan adanya peningkatan aktivitas katalase yang signifikan (p = 0,041), terutama antara kelompok K- dengan kelompok K+ (p = 0,032) dan antara kelompok Kp dengan kelompok K+ (p = 0,025). Simpulan penelitian menunjukkan bahwa ekstrak S. platensis terbukti memberikan pengaruh antioksidan terhadap peningkatan aktivitas katalase jaringan hepar tikus yang diinduksi parasetamol dosis tinggi.

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